CDK5 Protein (AA 1-292) (GST tag)
(1 reference)Quick Overview for CDK5 Protein (AA 1-292) (GST tag) (ABIN1348956)
Target
See all CDK5 ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-292
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Purification tag / Conjugate
- This CDK5 protein is labelled with GST tag.
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Purpose
- CDK5 (Human) Recombinant Protein (P01)
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Sequence
- MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR EICLLKELKH KNIVRLHDVL HSDKKLTLVF EFCDQDLKKY FDSCNGDLDP EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN VVPKLNATGR DLLQNLLKCN PVQRISAEEA LQHPYFSDFC PP
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Characteristics
- Human CDK5 full-length ORF ( NP_004926.1, 1 a.a. - 292 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Cyclin-Dependent Kinase 5 (CDK5) (AA 1-292) protein (His tag)
custom-made CDK5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin-Dependent Kinase 5 (CDK5) (AA 1-292) protein (His tag)custom-made CDK5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin-Dependent Kinase 5 (CDK5) (AA 1-292) protein (His tag)CDK5 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Cdk5-mediated phosphorylation of c-Myc on Ser-62 is essential in transcriptional activation of cyclin B1 by cyclin G1." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15601-10, (2008) (PubMed).
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: "Cdk5-mediated phosphorylation of c-Myc on Ser-62 is essential in transcriptional activation of cyclin B1 by cyclin G1." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15601-10, (2008) (PubMed).
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- CDK5 (Cyclin-Dependent Kinase 5 (CDK5))
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Alternative Name
- CDK5
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Background
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Full Gene Name: cyclin-dependent kinase 5
Synonyms: PSSALRE -
Gene ID
- 1020
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NCBI Accession
- NM_004935
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Pathways
- Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
Target
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