CHEK2 Protein (AA 1-543) (GST tag)

Catalog No. ABIN1349305

Product Details

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Protein Type: Recombinant

Protein Characteristics: AA 1-543

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This CHEK2 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: CHEK2 (Human) Recombinant Protein (P01)

Sequence: MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS QSSHSSSGTL SSLETVSTQE LYSIPEDQEP EDQEPEEPTP APWARLWALQ DGFANLECVN DNYWFGRDKS CEYCFDEPLL KRTDKYRTYS KKHFRIFREV GPKNSYIAYI EDHSGNGTFV NTELVGKGKR RPLNNNSEIA LSLSRNKVFV FFDLTVDDQS VYPKALRDEY IMSKTLGSGA CGEVKLAFER KTCKKVAIKI ISKRKFAIGS AREADPALNV ETEIEILKKL NHPCIIKIKN FFDAEDYYIV LELMEGGELF DKVVGNKRLK EATCKLYFYQ MLLAVQYLHE NGIIHRDLKP ENVLLSSQEE DCLIKITDFG HSKILGETSL MRTLCGTPTY LAPEVLVSVG TAGYNRAVDC WSLGVILFIC LSGYPPFSEH RTQVSLKDQI TSGKYNFIPE VWAEVSEKAL DLVKKLLVVD PKARFTTEEA LRHPWLQDED MKRKFQDLLS EENESTALPQ VLAQPSTSRK RPREGEAEGA ETTKRPAVCA AVL

Characteristics: Human CHEK2 full-length ORF ( AAH04207.1, 1 a.a. - 543 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Troncale, Barbet, Coulibaly, Henry, He, Barillot, Dubois, Hupé, de Koning: "NormaCurve: a SuperCurve-based method that simultaneously quantifies and normalizes reverse phase protein array data." in: PLoS ONE, Vol. 7, Issue 6, pp. e38686, (2012) (PubMed).

Yang, Chevolot, Géhin, Solassol, Mange, Souteyrand, Laurenceau: "Improvement of protein immobilization for the elaboration of tumor-associated antigen microarrays: application to the sensitive and specific detection of tumor markers from breast cancer sera." in: Biosensors & bioelectronics, Vol. 40, Issue 1, pp. 385-92, (2012) (PubMed).

Target Details

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2 (CHEK2 Products)

Background: Full Gene Name: CHK2 checkpoint homolog (S. pombe)
Synonyms: CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53

Gene ID: 11200

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle