GCK Protein (AA 1-465) (GST tag)
Quick Overview for GCK Protein (AA 1-465) (GST tag) (ABIN1354918)
Target
See all GCK ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-465
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Purification tag / Conjugate
- This GCK protein is labelled with GST tag.
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Purpose
- GCK (Human) Recombinant Protein (P01)
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Sequence
- MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
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Characteristics
- Human GCK full-length ORF ( AAH01890, 1 a.a. - 465 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)
custom-made GCK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)custom-made GCK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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Alternative Name
- GCK
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Background
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Full Gene Name: glucokinase (hexokinase 4)
Synonyms: GK,GLK,HHF3,HK4,HKIV,HXKP,MODY2 -
Gene ID
- 2645
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Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
Target
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