Granulin Protein (GRN) (AA 494-593) (GST tag)

Catalog No. ABIN1355792

Product Details

Target: Granulin (GRN)

Protein Type: Recombinant

Protein Characteristics: AA 494-593

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This Granulin protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: GRN (Human) Recombinant Protein (Q01)

Sequence: SCEKEVVSAQ PATFLARSPH VAVKDVECGE GHFCHDNQTC CRDNRQGWAC CPYRQGVCCA DRRHCCPAGF RCAARGTKCL RREAPRWDAP LRDPALRQLL

Characteristics: Human GRN partial ORF ( NP_002078, 494 a.a. - 593 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Chen, Chang, Deng, Xu, Nguyen, Martens, Cenik, Taylor, Hudson, Chung, Yu, Yu, Herz, Farese, Kukar, Tansey: "Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 21, pp. 9202-13, (2013) (PubMed).

De Muynck, Herdewyn, Beel, Scheveneels, Van Den Bosch, Robberecht, Van Damme: "The neurotrophic properties of progranulin depend on the granulin E domain but do not require sortilin binding." in: Neurobiology of aging, Vol. 34, Issue 11, pp. 2541-7, (2013) (PubMed).

Van Damme, Van Hoecke, Lambrechts, Vanacker, Bogaert, van Swieten, Carmeliet, Van Den Bosch, Robberecht: "Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival." in: The Journal of cell biology, Vol. 181, Issue 1, pp. 37-41, (2008) (PubMed).

Target Details

Target: Granulin (GRN)

Alternative Name: GRN (GRN Products)

Background: Full Gene Name: granulin
Synonyms: GEP,GP88,PCDGF,PEPI,PGRN

Gene ID: 2896

NCBI Accession: NM_002087