MANF Protein

Catalog No. ABIN1405920

Product Details

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Characteristics: Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke

Purification: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy.

Endotoxin Level: Less than 1EU/µg of protein as determined by LAL method

Application Details

Comment:

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP

Restrictions: For Research Use only

Handling

Concentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.4

Handling Advice: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Target Details

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF (MANF Products)

Synonyms: ARMET Protein, ARP Protein, 3230402M22Rik Protein, AA407711 Protein, AA408789 Protein, AA673178 Protein, Armet Protein, D17914 Protein, D18Mgi17 Protein, MANF Protein, RBM15B Protein, ARP-like Protein, CG7013 Protein, DmMANF Protein, Dmel\\CG7013 Protein, anon-EST:ParkEST188 Protein, arp Protein, armet Protein, im:6909481 Protein, zgc:153327 Protein, manf Protein, mesencephalic astrocyte derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor Protein, RNA binding motif protein 15B Protein, Mesencephalic astrocyte-derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor S homeolog Protein, MANF Protein, Manf Protein, RBM15B Protein, manf Protein, manf.S Protein

Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Molecular Weight: Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: P55145