PLGF Protein (Homodimer)

Catalog No. ABIN1589562

This Recombinant PLGF protein is expressed in Insect Cells.

Quick Overview for PLGF Protein (Homodimer) (ABIN1589562)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Insect Cells

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: Homodimer

Purpose: PlGF

Sequence: ALSAGNNSTE VEVVPFNEVW GRSYCRPMEK LVYILDEYPD EVSHIFSPSC VLLSRCSGCC GDEGLHCVPI KTANITMQIL KIPPNRDPHF YVEMTFSQDV LCECRPILET TKAERRKTKG KRKRSRNSQT EEPHP

Specificity: Chromosomal location:12 D, 12 39.0 cM

Characteristics: Length (aa):135/132

Application Details

Application Notes: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant mouse PlGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilised PlGF is supplied in lyophilized form with carrier-protein (BSA) and can be reconstituted with 50 mM acetic acid or PBS/water. This solution can be diluted into other buffered solutions or stored frozen for future use.

Buffer: 25 mM Tris, 75 mM NaCl pH 8.5

Storage: RT,-20 °C,-80 °C

Storage Comment: The lyophilized mouse PIGF, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Target Details

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: PlGF

Background: Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF shares 65 % amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
Synonyms: Pgf, PlGF, Plgf, AI854365, placental growth factor

Molecular Weight: ~40 kDa

Gene ID: 18654

NCBI Accession: NM_008827, NP_032853

UniProt: P49764

Pathways: VEGFR1 Specific Signals