CNTF Protein

Catalog No. ABIN1589602

Recombinant CNTF protein expressed in Escherichia coli (E. coli).

Quick Overview for CNTF Protein (ABIN1589602)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE

Product Details

Purpose: CNTF

Sequence: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Specificity: Chromosomal location:1q43

Characteristics: Length (aa):199

Endotoxin Level: < 0.1 ng per μg of CNTF

Application Details

Application Notes: Measured in a cell proliferation assay using TF1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140, 1989). The ED50 for this effect is typically 3 - 15 ng/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rat CNTF should be reconstituted in PBS to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffered solutions.

Buffer: 5 mM sodium acetate, pH 6.5

Storage: RT,-20 °C

Storage Comment: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted rat CNTF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Target Details

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. The cDNA for recombinant rat CNTF (Ala2–Met200) was cloned from total RNA of a rat embryo using standard protocols. Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. More recently, CNTF has been shown to promote survivability and differentiation of other neuronal cell types. Rat CNTF is a 22.7 kDa protein containing 199 amino acid residues.
Synonyms: Cntf

Molecular Weight: 22.7 kDa

Gene ID: 25707

NCBI Accession: NM_013166, NP_037298

UniProt: P20294

Pathways: JAK-STAT Signaling