PDGF-AB Heterodimer (Homodimer) (Active) Protein

Catalog No. ABIN1589627

This Recombinant PDGF-AB Heterodimer protein is produced in Escherichia coli (E. coli).

Quick Overview for PDGF-AB Heterodimer (Homodimer) (Active) Protein (ABIN1589627)

Target: PDGF-AB Heterodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: Homodimer

Purpose: Recombinant Human PDGF-AB Protein

Sequence: Alpha chain: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Specificity: Chromosomal location:7p22/22q13.1

Characteristics: Length (aa):126/110

Endotoxin Level: < 0.1 ng per ug of PDGF-AB

Application Details

Application Notes: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 mM acetic acid,Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.

Buffer: 50 mM acetic acid

Handling Advice: Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 2-3 weeks

Target Details

Target: PDGF-AB Heterodimer

Alternative Name: PDGF-AB

Background:

Synonyms: PDGFA, PDGF1, PDGF-A

Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).

Molecular Weight: 25.5 kDa

Gene ID: 5154, 5155

NCBI Accession: NM_002608, NM_002607, NP_002599, NP_002598

UniProt: P04085