IL-4 Protein

Catalog No. ABIN1589638

Recombinant IL-4 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-4 Protein (ABIN1589638)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE

Product Details

Purpose: IL-4

Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Specificity: Chromosomal location:5q31.1

Characteristics: Length (aa):130

Endotoxin Level: < 0.1 ng per μg of IL-4

Application Details

Application Notes: The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is 0.1-0.5 ng/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized IL-4 should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: PBS

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: RT,0 °C,4 °C,-20 °C

Storage Comment: The lyophilized IL-4, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-4 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 130 amino acid residues
Synonyms: IL4, BSF1, IL-4, BCGF1, BSF-1, BCGF-1

Molecular Weight: 14.9 kDa

Gene ID: 3565

NCBI Accession: NM_000589, NP_000580

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation