IL-3 Protein
Quick Overview for IL-3 Protein (ABIN1589649)
Target
See all IL-3 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- IL-3
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Sequence
- MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
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Specificity
- Chromosomal location:5q31.1
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Characteristics
- Length (aa):134
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Endotoxin Level
- < 0.1 ng per μg (IEU/μg) of rh IL-3
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 3 (IL-3) (AA 20-152) protein (Fc Tag)IL-3 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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Application Notes
- The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1 - 0.5 ng/mL. The WHO standard #91/510 was used as a control.
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Comment
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Cytokines & Growth Factors
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- The lyophilized IL-3 should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
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Buffer
- PBS
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Storage
- RT,0 °C,-20 °C
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Storage Comment
- The lyophilized IL-3, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-3 should be stored in working aliquots at -20°C.
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- IL-3 (Interleukin 3 (IL-3))
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Alternative Name
- IL-3
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Background
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IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.
Synonyms: IL3, IL-3, MCGF, MULTI-CSF -
Molecular Weight
- 15.0 kDa
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Gene ID
- 3562
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NCBI Accession
- NM_000588, NP_000579
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UniProt
- P08700
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Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target
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