HCP Protein

Catalog No. ABIN1607597

Supplier Product No.: 000-001-j08

This Native HCP protein is produced in Escherichia coli (E. coli).

Quick Overview for HCP Protein (ABIN1607597)

Target: HCP (Host Cell Proteins (HCP))

Protein Type: Native

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)

Product Details

Supplier Product No.: 000-001-j08

Supplier: Rockland

Purpose: E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)

Specificity: E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.

Characteristics: Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen

Application Details

Application Notes: Western Blot Dilution: 1:500 - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: HCP (Host Cell Proteins (HCP))

Alternative Name: Host Cell Proteins