Host Cell Proteins (HCP) Protein

Details for Product No. ABIN1607597
Protein Name
Origin
Escherichia coli (E. coli)
Source
Escherichia coli (E. coli)
Protein Type
Native
Application
ELISA, Western Blotting (WB)
Options
Purpose E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
Specificity E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
Characteristics Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
Protein Name
Application Notes Western Blot Dilution: 1:500 - 1:1000
Comment

E. coli Host Cell Protein Control Proteins detects over 300 E.coli proteins and is suitable for western blotting and ELISA. Kits are also useful in quality control applications as well as lot release testing. Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination.

Restrictions For Research Use only
Format Liquid
Concentration 4.0 mg/mL
Buffer 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
Preservative Without preservative
Storage -20 °C
Storage Comment Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiry Date 12 months
Supplier Images
 image for Host Cell Proteins (HCP) protein (ABIN1607597) 2D Oriole Stain of E.coli HCP Control Protein. Load: 35ug Total HCP. pH: 5-8. Exposur...
SDS-PAGE (SDS) image for Host Cell Proteins (HCP) protein (ABIN1607597) SDS-PAGE results of E.coli Host Cell Protein Control Protein. Lane 1: reduced E. coli...
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