PCMT1 Protein (AA 2-227) (His tag)

Catalog No. ABIN1633923

This Recombinant PCMT1 protein is expressed in Yeast.

Quick Overview for PCMT1 Protein (AA 2-227) (His tag) (ABIN1633923)

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Protein Type: Recombinant

Origin: Orang-Utan

Source: Yeast

Application: ELISA

Purity: > 90 %

Product Details

Protein Characteristics: AA 2-227

Purification tag / Conjugate: This PCMT1 protein is labelled with His tag.

Sequence: GWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKCNPYMDSP QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV GCTGKVIGID HIKELVDDSI NNVRKDDPTL LSSGRVQLVV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD GSVKMKPLMG VIYVPLTDKE KQWSRWK

Specificity: Pongo abelii (Sumatran orangutan)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Background: Recommended name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase.
Short name= PIMT.
EC= 2.1.1.77.
Alternative name(s): L-isoaspartyl protein carboxyl methyltransferase Protein L-isoaspartyl/D-aspartyl methyltransferase Protein-beta-aspartate methyltransferase

UniProt: Q5RA89

Pathways: Ribonucleoprotein Complex Subunit Organization