Quick Overview for PCMT1 Protein (Myc-DYKDDDDK Tag) (ABIN2728412)
Target
See all PCMT1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PCMT1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PCMT1 / PIMT protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 1-228) protein (GST tag)
PCMT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 1-227) protein (His tag)PCMT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))
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Alternative Name
- Pcmt1,pimt
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Background
- This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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Molecular Weight
- 30.1 kDa
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NCBI Accession
- NP_005380
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Pathways
- Ribonucleoprotein Complex Subunit Organization
Target
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