Leptin Receptor Protein (LEPR) (C-Term, Extracellular Domain)

Catalog No. ABIN1684639

This Recombinant Leptin Receptor protein is produced in HEK-293T Cells.

Quick Overview for Leptin Receptor Protein (LEPR) (C-Term, Extracellular Domain) (ABIN1684639)

Target: Leptin Receptor (LEPR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293T Cells

Purity: > 98 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of human leptin receptor including a C-terminal Human IgG1 Fc tag was expressed in HEK293 cells.

Characteristics: Recombinant human leptin receptor is a homodimer protein consisting of two 1070 amino acid residue subunit chains, due to glycosylation migrates as an approximately 160kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

Biologically active: Measured by its ability to bind immobilized recombinant human Leptin (1 µg/mL) at a linear concentration range of 0.01-4 µg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS solution, pH7.2

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.

Expiry Date: 12 months

Target Details

Target: Leptin Receptor (LEPR)

Alternative Name: LEPR

Background: FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre-adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

UniProt: P48357

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour