LYPLA1 Protein (AA 1-230) (His tag)
Quick Overview for LYPLA1 Protein (AA 1-230) (His tag) (ABIN1690840)
Target
See all LYPLA1 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-230
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Purification tag / Conjugate
- This LYPLA1 protein is labelled with His tag.
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Purpose
- Recombinant Human Acyl-Protein Thioesterase 1/APT-1 (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MCGNNMSTPL PAIVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIRSSHIK YICPHAPVRP VTLNMNVAMP SWFDIIGLSP DSQEDESGIK QAAENIKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFPQGPIGGA NRDISILQCH GDCDPLVPLM FGSLTVEKLK TLVNPANVTF KTYEGMMHSS CQQEMMDVKQ FIDKLLPPID
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Characteristics
- Recombinant Human APT-1/LYPLA1 produced by E. coli expression system. Target protein is expressed with sequence (Met1-Asp230) of Human LYPLA1 (Uniprot #O75608) fused with a His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Lysophospholipase I (LYPLA1) (AA 1-230) protein (His tag)
custom-made LYPLA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase I (LYPLA1) (AA 1-230) protein (His tag)custom-made LYPLA1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase I (LYPLA1) (AA 1-230) protein (GST tag)LYPLA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Comment
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Gene Name: LYPLA1 APT1 LPL1
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- LYPLA1 (Lysophospholipase I (LYPLA1))
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Alternative Name
- Acyl-Protein Thioesterase 1/APT-1
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Molecular Weight
- 26.83 kDa
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UniProt
- O75608
Target
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