LYPLA1 Protein (His tag)
Quick Overview for LYPLA1 Protein (His tag) (ABIN7318133)
Target
See all LYPLA1 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This LYPLA1 protein is labelled with His tag.
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Purpose
- Recombinant Human LYPLA1 Protein (His Tag)
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Sequence
- Met 1-Asp230
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Characteristics
- Recombinant Human Acyl-Protein Thioesterase 1 is produced by our E.coli expression system and the target gene encoding Met1-Asp230 is expressed with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Lysophospholipase I (LYPLA1) (AA 1-230) protein (His tag)
custom-made LYPLA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase I (LYPLA1) (AA 1-230) protein (His tag)custom-made LYPLA1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase I (LYPLA1) (AA 1-230) protein (His-IF2DI Tag)LYPLA1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lysophospholipase I (LYPLA1) (AA 1-230) protein (GST tag)LYPLA1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- LYPLA1 (Lysophospholipase I (LYPLA1))
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Alternative Name
- LYPLA1
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Background
- APT-1,APT1,Acyl-Protein Thioesterase 1,LPL-I,LPL1,LYPLA1,LysoPLA I,Lysophospholipase 1,Lysophospholipase I,hAPT1,Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS, in addition, it also has depalmitoylating activity and low lysophospholipase activity.
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Molecular Weight
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Calculated MW: 26.8 kDa
Observed MW: 25 kDa
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Gene ID
- 10434
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UniProt
- O75608
Target
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