OGG1 Protein (AA 1-345) (GST tag)

Catalog No. ABIN1690871

This Recombinant OGG1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for OGG1 Protein (AA 1-345) (GST tag) (ABIN1690871)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-345

Purification tag / Conjugate: This OGG1 protein is labelled with GST tag.

Purpose: Recombinant Human N-Glycosylase/OGG1 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMPAR ALLPRRMGHR TLASTPALWA SIPCPRSELR LDLVLPSGQS FRWREQSPAH WSGVLADQVW TLTQTEEQLH CTVYRGDKSQ ASRPTPDELE AVRKYFQLDV TLAQLYHHWG SVDSHFQEVA QKFQGVRLLR QDPIECLFSF ICSSNNNIAR ITGMVERLCQ AFGPRLIQLD DVTYHGFPSL QALAGPEVEA HLRKLGLGYR ARYVSASARA ILEEQGGLAW LQQLRESSYE EAHKALCILP GVGTKVADCI CLMALDKPQA VPVDVHMWHI AQRDYSWHPT TSQAKGPSPQ TNKELGNFFR SLWGPYAGWA QAVLFSADLR QSRHAQEPPA KRRKGSKGPE G

Characteristics: Recombinant Human N-Glycosylase/DNA Lyase is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gly345) of Human DNA Lyase fused with a GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: N-Glycosylase/DNA Lyase

Sub Type: Fusionprotein

Background: Human N-Glycosylase/DNA Lyase is a DNA repair enzyme, which incises DNA at 8-oxoG residues, and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damage DNA. DGG1 has a beta-lyase activity that nicks DNA 3’ to the lesion.
Alternative Names: N-Glycosylase/DNA Lyase, 8-Oxoguanine DNA Glycosylase, DNA-(Apurinic or Apyrimidinic Site) Lyase, AP Lyase, OGG1, MMH, MUTM, OGH1

Molecular Weight: 65.07 kDa

UniProt: O15527

Pathways: DNA Damage Repair