CST7 Protein (AA 20-145) (His tag)

Catalog No. ABIN1691071

This Recombinant CST7 protein is produced in Human Cells.

Quick Overview for CST7 Protein (AA 20-145) (His tag) (ABIN1691071)

Target: CST7 (Cystatin F (Leukocystatin) (CST7))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 20-145

Purification tag / Conjugate: This CST7 protein is labelled with His tag.

Purpose: Recombinant Human Cystatin F/CST7 (C-6His)

Sequence: GPSPDTCSQD LNSRVKPGFP KTIKTNDPGV LQAARYSVEK FNNCTNDMFL FKESRITRAL VQIVKGLKYM LEVEIGRTTC KKNQHLRLDD CDFQTNHTLK QTLSCYSEVW VVPWLQHFEV PVLRCHVDHH HHHH

Characteristics:

Recombinant Human Cystatin F/CST7 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CST7 (Cystatin F (Leukocystatin) (CST7))

Alternative Name: Cystatin-F

Background: Recombinant Human Cystatin-F is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (G20-H145) of Human CST7 fused with a polyhistidine tag at the C-terminus.
CST7 is a secreted protein and primarily expressed in peripheral blood cells and spleen.It is belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system.

Molecular Weight: 15.58 kDa

UniProt: O76096