CST7 Protein (Fc Tag)
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- Target See all CST7 Proteins
- CST7 (Cystatin F (Leukocystatin) (CST7))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CST7 protein is labelled with Fc Tag.
- Purpose
- Recombinant Mouse Cystatin 7/CST7 Protein (Fc Tag)(Active)
- Sequence
- Met1-Gln144
- Characteristics
- A DNA sequence encoding the mouse CST7 (O89098) (Met1-Gln144) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
- Purity
- > 93 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 6 nM.
- Top Product
- Discover our top product CST7 Protein
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Cystatin F (Leukocystatin) (CST7) (AA 68-167) protein (GST tag)
CST7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cystatin F (Leukocystatin) (CST7) (AA 1-167) protein (GST tag)CST7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cystatin F (Leukocystatin) (CST7) protein (His tag)CST7 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Cystatin F (Leukocystatin) (CST7) (AA 20-145) protein (His tag)Crystallography grade CST7 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Cystatin F (Leukocystatin) (CST7) (AA 19-144) protein (His tag)Crystallography grade CST7 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 25 mM Tris, 0.15M NaCl, pH 7.5
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CST7 (Cystatin F (Leukocystatin) (CST7))
- Alternative Name
- Cystatin 7/CST7 (CST7 Products)
- Synonyms
- CST7 Protein, LOC100232568 Protein, cytf Protein, CMAP Protein, Cmap Protein, cystatin F Protein, Cystatin F Protein, cystatin F (leukocystatin) Protein, CST7 Protein, cytf Protein, Cst7 Protein
- Background
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Background: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.
Synonym: Cystatin-F, Cystatin-like Metastasis-Associated Protein, Leukocystatin, CMAP, Cystatin-7, Cst7
- Molecular Weight
- 41.4 kDa
- UniProt
- O89098
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