CDH6 Protein (AA 22-615) (His tag)

Catalog No. ABIN1691153

This Recombinant CDH6 protein is produced in Human Cells.

Quick Overview for CDH6 Protein (AA 22-615) (His tag) (ABIN1691153)

Target: CDH6 (K-Cadherin (CDH6))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-615

Purification tag / Conjugate: This CDH6 protein is labelled with His tag.

Purpose: Recombinant Human Cadherin-6/K-Cadherin/CDH6 (C-6His)

Sequence: TPLSKRTSGF PAKKRALELS GNSKNELNRS KRSWMWNQFF LLEEYTGSDY QYVGKLHSDQ DRGDGSLKYI LSGDGAGDLF IINENTGDIQ ATKRLDREEK PVYILRAQAI NRRTGRPVEP ESEFIIKIHD INDNEPIFTK EVYTATVPEM SDVGTFVVQV TATDADDPTY GNSAKVVYSI LQGQPYFSVE SETGIIKTAL LNMDRENREQ YQVVIQAKDM GGQMGGLSGT TTVNITLTDV NDNPPRFPQS TYQFKTPESS PPGTPIGRIK ASDADVGENA EIEYSITDGE GLDMFDVITD QETQEGIITV KKLLDFEKKK VYTLKVEASN PYVEPRFLYL GPFKDSATVR IVVEDVDEPP VFSKLAYILQ IREDAQINTT IGSVTAQDPD AARNPVKYSV DRHTDMDRIF NIDSGNGSIF TSKLLDRETL LWHNITVIAT EINNPKQSSR VPLYIKVLDV NDNAPEFAEF YETFVCEKAK ADQLIQTLHA VDKDDPYSGH QFSFSLAPEA ASGSNFTIQD NKDNTAGILT RKNGYNRHEM STYLLPVVIS DNDYPVQSST GTVTVRVCAC DHHGNMQSCH AEALIHPTGL STGAVDHHHH HH

Characteristics:

Recombinant Human Cadherin-6/K-Cadherin/CDH6 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: Cadherin-6

Background: Recombinant Human Cadherin-6 produced by transfected human cells is a secreted protein with sequence (Ser54-Ala615) of Human CDH6 fused with a polyhistidine tag at the C-terminus.
Cadherin-6 (CDH6) is a type-II classic cadherin cell-cell adhesion molecules, which are expressed in graded or areal patterns, as well as layer-specific patterns, in the cortical plate. Human Cadherin-6 is synthesized as a 790 aa type I transmembrane glycoprotein that contains a 18 aa signal peptide, a 35 aa propeptide, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain. There are five cadherin domains of approximately 110 aa each in the extracellular region. Cadherin-6 has high expression in kidney, brain, and cerebellum, and may contribute to the formation of the segmental structure of the early brain, as well as the development of renal proximal tubules. Weak expression is also detected in lung, pancreas, gastric mucosa and cytotrophoblasts. As a classic cadherin, Cadherin-6 will form homodimers and promote intercellular adhesion with itself and, possibly, Cadherin-9 and -14.

Molecular Weight: 67.43 kDa

UniProt: P55285

Pathways: Cell-Cell Junction Organization