RCN3 Protein (AA 21-328) (His tag)

Catalog No. ABIN1691419

This Recombinant RCN3 protein is produced in Human Cells.

Quick Overview for RCN3 Protein (AA 21-328) (His tag) (ABIN1691419)

Target: RCN3 (Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-328

Purification tag / Conjugate: This RCN3 protein is labelled with His tag.

Purpose: Recombinant Human Reticulocalbin-3/RCN3 (C-6His)

Sequence: KPSPDAGPHG QGRVHQAAPL SDAPHDDAHG NFQYDHEAFL GREVAKEFDQ LTPEESQARL GRIVDRMDRA GDGDGWVSLA ELRAWIAHTQ QRHIRDSVSA AWDTYDTDRD GRVGWEELRN ATYGHYAPGE EFHDVEDAET YKKMLARDER RFRVADQDGD SMATREELTA FLHPEEFPHM RDIVIAETLE DLDRNKDGYV QVEEYIADLY SAEPGEEEPA WVQTERQQFR DFRDLNKDGH LDGSEVGHWV LPPAQDQPLV EANHLLHESD TDKDGRLSKA EILGNWNMFV GSQATNYGED LTRHHDELVD HHHHHH

Characteristics:

Recombinant Human Reticulocalbin-3/RCN3 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,10 % Glycerol, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: RCN3 (Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3))

Alternative Name: Reticulocalbin-3

Background: Recombinant Human Reticulocalbin-3/RCN3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Lys21-Leu328) of Human RCN3 fused with a polyhistidine tag at the C-terminus.
Reticulocalbin-3 is a member of the CREC family that is involved in the secretory pathway. Members of the CREC family consist of a number of multiple EF-hand domains. Reticulocalbin-3 localizes to the endoplasmic reticulum lumen. The bioinformaticanalysis of Reticulocalbin-3 reveal that it is a putative Ca2+-binding protein. In addition, it has been shown that autoactivation and secretion of PACE4 was increased upon co-expression with Reticulocalbin-3. The proPACE4-RCN-3 complex is plays an important role in the biosynthesis of PACE4.

Molecular Weight: 36.2 kDa

UniProt: Q96D15