RNASE6 Protein (AA 24-150) (His tag)

Catalog No. ABIN1691431

This Recombinant RNASE6 protein is produced in Human Cells.

Quick Overview for RNASE6 Protein (AA 24-150) (His tag) (ABIN1691431)

Target: RNASE6 (Ribonuclease, RNase A Family, K6 (RNASE6))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 24-150

Purification tag / Conjugate: This RNASE6 protein is labelled with His tag.

Purpose: Recombinant Human Ribonuclease K6/RNASE6 (C-6His)

Sequence: WPKRLTKAHW FEIQHIQPSP LQCNRAMSGI NNYTQHCKHQ NTFLHDSFQN VAAVCDLLSI VCKNRRHNCH QSSKPVNMTD CRLTSGKYPQ CRYSAAAQYK FFIVACDPPQ KSDPPYKLVP VHLDSILVDH HHHHH

Characteristics:

Recombinant Human Ribonuclease K6/RNASE6 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mMDTT,10 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: RNASE6 (Ribonuclease, RNase A Family, K6 (RNASE6))

Alternative Name: Ribonuclease K6/RNASE6

Background: Recombinant Human Ribonuclease K6/RNASE6 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Trp24-Leu150) of Human RNASE6 fused with a polyhistidine tag at the C-terminus.
Ribonuclease K6 (RNASE6) is a secreted protein that belongs to the pancreatic ribonuclease family. Human RNASE6 is synthesized as a 150 AA precursor that contains a 23 AA signal sequence, and a 127 AA mature chain. RNASE6 is expressed in many tissues, with high expression levels in the lung, with lower expression levels in the heart, placenta, kidney, pancreas, liver, brain, and skeletal muscle. It is also detected in monocytes and neutrophils. RNASE6 may have a role in host defense.

Molecular Weight: 15.69 kDa

UniProt: Q93091