Cathepsin L Protein (AA 18-334) (His tag)

Catalog No. ABIN1691578

This Recombinant Cathepsin L protein is produced in Human Cells.

Quick Overview for Cathepsin L Protein (AA 18-334) (His tag) (ABIN1691578)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 18-334

Purification tag / Conjugate: This Cathepsin L protein is labelled with His tag.

Purpose: Recombinant Mouse Cathepsin L/CTSL (C-6His)

Sequence: TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQ EPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLD SEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEG TDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVNVDH HHHHH

Characteristics: Recombinant Mouse Cathepsin L/Ctsl1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Thr18-Asn334) of Mouse Cathepsin L fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 6 months

Target Details

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L

Sub Type: Fusionprotein

Background: Mouse Cathepsin L is a lysosomal cysteine proteinase which is a member of the peptidase C1 family. This protein is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsin L plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. Cathepsin L has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria.
Alternative Names: Cathepsin L1, Major excreted protein, p39 cysteine proteinase, Ctsl1,

Molecular Weight: 36.8 kDa

UniProt: P06797

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades