Cathepsin L Protein (AA 18-334) (His tag)
Quick Overview for Cathepsin L Protein (AA 18-334) (His tag) (ABIN6387773)
Target
See all Cathepsin L (CTSL1) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 18-334
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Purification tag / Conjugate
- This Cathepsin L protein is labelled with His tag.
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Sequence
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TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQ EPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLD SEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEG TDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVN
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Cathepsin L1 (CTSL1) (AA 1-333) protein (GST tag)
CTSL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin L1 (CTSL1) (AA 18-333) protein (GST tag)CTSL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin L1 (CTSL1) (AA 18-333) protein (His-IF2DI Tag)CTSL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cathepsin L1 (CTSL1) (AA 1-334) protein (His tag)CTSL1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
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Alternative Name
- Cathepsin L
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Background
- CTSL, also known as cathepsin L1, is a lysosomal cysteine protease expressed in most eukaryotic cells. It plays a major role in intracellular protein catabolism and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. Recombinant mouse CTSL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 36.8 kDa (325aa)
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NCBI Accession
- NP_034114
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Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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