LRG1 Protein (AA 36-347) (His tag)

Catalog No. ABIN1691794

Recombinant LRG1 protein expressed in Human Cells.

Quick Overview for LRG1 Protein (AA 36-347) (His tag) (ABIN1691794)

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 36-347

Purification tag / Conjugate: This LRG1 protein is labelled with His tag.

Purpose: Recombinant Human Leucine-Rich α-2-Glycoprotein/LRG1 (C-6His)

Sequence: VTLSPKDCQV FRSDHGSSIS CQPPAEIPGY LPADTVHLAV EFFNLTHLPA NLLQGASKLQ ELHLSSNGLE SLSPEFLRPV PQLRVLDLTR NALTGLPPGL FQASATLDTL VLKENQLEVL EVSWLHGLKA LGHLDLSGNR LRKLPPGLLA NFTLLRTLDL GENQLETLPP DLLRGPLQLE RLHLEGNKLQ VLGKDLLLPQ PDLRYLFLNG NKLARVAAGA FQGLRQLDML DLSNNSLASV PEGLWASLGQ PNWDMRDGFD ISGNPWICDQ NLSDLYRWLQ AQKDKMFSQN DTRCAGPEAV KGQTLLAVAK SQVDHHHHHH

Characteristics:

Recombinant Human Leucine-Rich α-2-Glycoprotein/LRG1 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 20 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Alternative Name: Leucine-Rich alpha-2-Glycoprotein/LRG1

Background: Recombinant Human Leucine-rich α-2-glycoprotein/LRG1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Val36-Gln347) of Human LRG1 fused with a polyhistidine tag at the C-terminus.
Leucine-rich α-2-glycoprotein is a secreted protein and contains 8 LRR (leucine-rich) repeats and 1 LRRCT domain. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation. Levels of the LRG protein are markedly elevated in acute appendicitis and therefore could be used as a diagnostic aid.

Molecular Weight: 35.3 kDa

UniProt: P02750

Pathways: Brown Fat Cell Differentiation