Deoxyuridine Triphosphatase (DUT) (AA 1-164) Protein

Catalog No. ABIN1692033

Recombinant Deoxyuridine Triphosphatase protein expressed in Escherichia coli (E. coli).

Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 1-164) Protein (ABIN1692033)

Target: Deoxyuridine Triphosphatase (DUT)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-164

Purpose: Recombinant Human dUTP Pyrophosphatase/dUTPase

Sequence: MPCSEETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN

Characteristics: Recombinant Human Deoxyuridine 5'-Triphosphate Nucleotidohydrolase,Mitochondrial is produced by our E.coli expression system and the target gene encoding Met1-Asn164 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of PBS, pH 7.4.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: Dutpase

Target Type: Viral Protein

Background: Alternative Names: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial, dUTPase, dUTP Pyrophosphatase, DUTDeoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial (dUTPase) belongs to the dUTPase family. dUTPase exits as a homotrimer and is involved in nucleotide metabolism. dUTPase produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair.
Alternative Names: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial, dUTPase, dUTP Pyrophosphatase, DUT

Molecular Weight: 17.7 kDa

UniProt: P33316-2