RPE Protein (AA 1-228) (His tag)

Catalog No. ABIN1692039

This Recombinant RPE protein is expressed in Escherichia coli (E. coli).

Quick Overview for RPE Protein (AA 1-228) (His tag) (ABIN1692039)

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-228

Purification tag / Conjugate: This RPE protein is labelled with His tag.

Purpose: Recombinant Human Ribulose-Phosphate 3-Epimerase/RPE (C-6His)

Sequence: MASGCKIGPS ILNSDLANLG AECLRMLDSG ADYLHLDVMD GHFVPNITFG HPVVESLRKQ LGQDPFFDMH MMVSKPEQWV KPMAVAGANQ YTFHLEATEN PGALIKDIRE NGMKVGLAIK PGTSVEYLAP WANQIDMALV MTVEPGFGGQ KFMEDMMPKV HWLRTQFPSL DIEVDGGVGP DTVHKCAEAG ANMIVSGSAI MRSEDPRSVI NLLRNVCSEA AQKRSLDRVD HHHHHH

Characteristics: Recombinant Human Ribulose-Phosphate 3-Epimerase/RPE is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg228) of Human RPE fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 6.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Alternative Name: RPE

Sub Type: Fusionprotein

Background: Ribulose-Phosphate 3-Epimerase (RPE) is a member of the Ribulose-Phosphate 3-Epimerase family. RPE exists as a homodimer and catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. RPE binds one divalent metal cation per subunit and contains tightly bound Fe2+ when produced in E. coli, but the physiological cofactor may be Co2+, Mn2+ or Zn2+. It has been shown that RPE participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation.
Alternative Names: Ribulose-Phosphate 3-Epimerase, Ribulose-5-Phosphate-3-Epimerase, RPE, HUSSY-17

Molecular Weight: 25.9 kDa