RPE Protein (His tag)

Catalog No. ABIN7317450

This Recombinant RPE protein is produced in HEK-293 Cells.

Quick Overview for RPE Protein (His tag) (ABIN7317450)

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This RPE protein is labelled with His tag.

Purpose: Recombinant Human RPE Protein (His Tag)

Sequence: Ala 2-Arg 228

Characteristics: A DNA sequence encoding the human RPE isoform 1 (NP_954699.1) (Ala 2-Arg 228) was expressed, with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))

Alternative Name: RPE

Background: HUSSY-17,RPE,RPE2-1,Ribulose-5-Phosphate-3-Epimerase,Ribulose-Phosphate 3-Epimerase,The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylase (OMPDC), and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. In enzymology, a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence, RPE has one substrate, L-ribulose 5-phosphate, and one product, L-xylulose 5-phosphate. RPE belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase, UlaE, and SgaU.

Molecular Weight:

Calculated MW: 27.0 kDa

Observed MW: 30 kDa

Gene ID: 6120

NCBI Accession: NP_954699

UniProt: Q96AT9