CCL18 Protein (AA 21-89) (His tag)

Catalog No. ABIN1692043

This Recombinant CCL18 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL18 Protein (AA 21-89) (His tag) (ABIN1692043)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-89

Purification tag / Conjugate: This CCL18 protein is labelled with His tag.

Purpose: Recombinant Human C-C Motif Chemokine 18/CCL18/PARC (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAQVGTNKEL CCLVYTSWQI PQKFIVDYSE TSPQCPKPGV ILLTKRGRQI CADPNKKWVQ KYISDLKLNA

Characteristics:

Recombinant Human C-C Motif Chemokine 18/CCL18/PARC (N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: C-C Motif Chemokine 18/CCL18

Background: Recombinant Human C-C Motif Chemokine 18/CCL18 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala21-Ala89) of Human CCL18 fused with a 6His tag at the N-terminus.
C-C Motif Chemokine 18 (CCL18) is secreted protein that belongs to the intercrine β (chemokine CC) family. CCL18 is expressed at high levels in the lung, lymph nodes, placenta, bone marrow, and dendritic cells. CCL18 is a chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. CCL18 may be involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.

Molecular Weight: 10.1 kDa

UniProt: P55774