CA14 Protein (AA 19-290) (His tag)

Catalog No. ABIN1692279

This Recombinant CA14 protein is produced in Escherichia coli (E. coli).

Quick Overview for CA14 Protein (AA 19-290) (His tag) (ABIN1692279)

Target: CA14 (Carbonic Anhydrase XIV (CA14))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-290

Purification tag / Conjugate: This CA14 protein is labelled with His tag.

Purpose: Recombinant Human Carbonic Anhydrase 14/CA14 (N-6His)

Sequence: MNHKVHHHHH HMGQHWTYEG PHGQDHWPAS YPECGNNAQS PIDIQTDSVT FDPDLPALQP HGYDQPGTEP LDLHNNGHTV QLSLPSTLYL GGLPRKYVAA QLHLHWGQKG SPGGSEHQIN SEATFAELHI VHYDSDSYDS LSEAAERPQG LAVLGILIEV GETKNIAYEH ILSHLHEVRH KDQKTSVPPF NLRELLPKQL GQYFRYNGSL TTPPCYQSVL WTVFYRRSQI SMEQLEKLQG TLFSTEEEPS KLLVQNYRAL QPLNQRMVFA SFIQAGSSYT TGEMVDLQSR

Characteristics:

Recombinant Human Carbonic Anhydrase 14/CA14 (N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CA14 (Carbonic Anhydrase XIV (CA14))

Alternative Name: Carbonic Anhydrase 14/CA14

Background: Recombinant Human Carbonic Anhydrase 14/CA14 is produced by our E. coli expression system. The target protein is expressed with sequence (Gly19-Met290) of Human CA14 fused with a 6His tag at the N-terminus.
Carbonic Anhydrase 14 (CA14) belongs to the α-Carbonic Anhydrase family. It is highly expressed in all parts of the central nervous system and lowly expressed in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle. CA14 along with other Carbonic Anhydrases (CAs) participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA14 is predicted to be a type I membrane protein and catalyzes the reversible hydration of carbon dioxide.

Molecular Weight: 32.8 kDa

UniProt: Q9ULX7