RHEB Protein (AA 1-184) (GST tag)

Catalog No. ABIN1692308

This Recombinant RHEB protein is expressed in Escherichia coli (E. coli).

Quick Overview for RHEB Protein (AA 1-184) (GST tag) (ABIN1692308)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-184

Purification tag / Conjugate: This RHEB protein is labelled with GST tag.

Purpose: Recombinant Human GTP-Binding Protein Rheb/RHEB (N-GST)

Sequence: MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF RRIILEAEKM DGAASQGKSS CSVM

Characteristics:

Recombinant Human GTP-Binding Protein Rheb/RHEB (N-GST)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 10 mM GSH, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: GTP-Binding Protein Rheb/RHEB

Background: Recombinant Human GTP-Binding Protein Rheb/RHEB is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met184) of Human RHEB fused with a GST tag at the N-terminus.
GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle, and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling, and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, farnesylation of the protein is required for this activity.

Molecular Weight: 20.4 kDa

UniProt: Q15382

Pathways: RTK Signaling