Chemokine (C-C Motif) Ligand 21A (Serine) (CCL21A) (AA 24-133) Protein

Details for Product No. ABIN1692325
Protein Name
Protein Characteristics
AA 24-133
3
Origin
Mouse (Murine)
6
Source
Escherichia coli (E. coli)
5
1
Protein Type
Recombinant
Application
Functional Studies (Func)
Options
Purpose Recombinant Mouse C-C Motif Chemokine 21a/CCL21a//6Ckine
Sequence SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FSPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
Characteristics

Recombinant Mouse C-C Motif Chemokine 21a/CCL21a//6Ckine

Purity > 95 % as determined by reducing SDS-PAGE.
Sterility 0.2 μm filtered
Endotoxin Level Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Protein Name
Background Recombinant Mouse C-C Motif Chemokine 21a/CCL21a is produced with our E. coli expression system. The target protein is expressed with sequence (Ser24-Gly133) of Mouse CCL21a.
C-C Motif Chemokine 21a (CCL21a) is a small secreted cytokine that belongs to the intercrine β (CC chemokine) family. Mouse CCL21 cDNA encodes a 133 AA residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Mouse CCL21 has three forms while CCL21a has Ser-65. CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7 and CXCR3. Mouse CCL21 inhibits hemopoiesis and stimulates chemotaxis. It has chemotactic function in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Mouse CCL21 shows preferential activity towards naive T-cells and may play a role in mediating homing of lymphocytes to secondary lymphoid organs.
Molecular Weight 12 kDa
UniProt P84444
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
Handling Advice Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Storage 4 °C/-20 °C/-80 °C
Storage Comment Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.