PIN4 Protein (AA 1-156) (His tag)
Quick Overview for PIN4 Protein (AA 1-156) (His tag) (ABIN1692384)
Target
See all PIN4 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-156
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Purification tag / Conjugate
- This PIN4 protein is labelled with His tag.
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Purpose
- Recombinant Human Parvulin-14/PIN4 (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK
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Characteristics
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Recombinant Human Parvulin-14/PIN4 (N-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4) (AA 1-131) protein (His tag)
custom-made PIN4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4) (AA 1-131) protein (His tag)custom-made PIN4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4) (AA 1-156) protein (His-IF2DI Tag)PIN4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4) (AA 1-131) protein (His-IF2DI Tag)PIN4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4) (AA 1-156) protein (GST tag)PIN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- PIN4 (Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4))
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Alternative Name
- Parvulin-14/PIN4
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Background
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Recombinant Human Parvulin-14/PIN4 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys156) of Human PIN4 fused with a 6His tag at the N-terminus.
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4(PIN4) is a peptidyl-prolyl cis/trans isomerase (PPIase) which interacts with NIMA and is vital for cell cycle regulation. PIN4 has 2 different isoforms: PAR14 and PAR17. Furthermore, PIN4 protein binds to double-stranded DNA under physiological salt conditions. PIN4 is involved as a ribosomal RNA processing factor in ribosome biogenesis. The PAR14 binds to tightly bent AT-rich stretches of double-stranded DNA, but PAR17 binds to double-stranded DNA. -
Molecular Weight
- 18.8 kDa
Target
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