MGMT Protein (AA 1-207) (His tag)
Quick Overview for MGMT Protein (AA 1-207) (His tag) (ABIN1692533)
Target
See all MGMT ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-207
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Purification tag / Conjugate
- This MGMT protein is labelled with His tag.
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Purpose
- Recombinant Human 6-O-Methylguanine-DNA Methyltransferase/MGMT (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
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Characteristics
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Recombinant Human 6-O-Methylguanine-DNA Methyltransferase/MGMT (N-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-211) protein (His tag)custom-made MGMT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mMTrisHCl,1 mM EDTA, pH 8.0 .
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- Methylated-DNA--Protein-Cysteine Methyltransferase/MGMT
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Background
- Recombinant Human Methylated-DNA--protein-cysteine methyltransferase/MGMT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn207) of Human MGMT fused with a 6His tag at the N-terminus.
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Molecular Weight
- 23.8 kDa
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UniProt
- P16455
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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