GOT1 Protein (AA 2-413) (His tag)

Catalog No. ABIN1879343

This Recombinant GOT1 protein is produced in Yeast (Pichia pastoris).

Quick Overview for GOT1 Protein (AA 2-413) (His tag) (ABIN1879343)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Protein Type: Recombinant

Origin: Human

Source: Yeast (Pichia pastoris)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purity: > 95 %

Product Details

Protein Characteristics: AA 2-413

Purification tag / Conjugate: This GOT1 protein is labelled with His tag.

Sequence: YVEFHHHHHH -APPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ

Characteristics: Location of tag(s): N-terminal

Application Details

Comment:

Subcellular Location: Cytoplasm
Predicted Isoelectric Point: 6.6

The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 °C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS, pH 7.2 - pH 7.4.

Buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5 % trehalose, 0.05 % sarcosyl.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Aspartate Aminotransferase (AST)

Molecular Weight: 47.5 kDa

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process