FGF4 Protein (AA 31-206)

Catalog No. ABIN2017823

Recombinant FGF4 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF4 Protein (AA 31-206) (ABIN2017823)

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Product Details

Protein Characteristics: AA 31-206

Characteristics: ED50<0.5 ng/mL, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >2.0× 10^6 units/mg
AA 31-206, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM HEPES, 750 mM NaCl, pH 7.5.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Expiry Date: 6 months

Target Details

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Alternative Name: Fibroblast Growth Factor-4 (FGF-4)

Background: Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis. Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E. coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques, rhFGF-4 has a molecular mass of 19.4 kDa as analyzed by reducing SDS-PAGE.
Synonyms: HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF.

Molecular Weight: 19.4kDa, observed by reducing SDS-PAGE.

UniProt: P08620

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance