FGF6 Protein (AA 41-208)

Catalog No. ABIN2017827

Recombinant FGF6 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF6 Protein (AA 41-208) (ABIN2017827)

Target: FGF6 (Fibroblast Growth Factor 6 (FGF6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 41-208

Characteristics: ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence 1 xg/mL heparin, corresponding to a specific activity of > 4x 10^5 units/mg.
AA 41-208, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF6 (Fibroblast Growth Factor 6 (FGF6))

Alternative Name: Fibroblast Growth Factor-6 (FGF-6)

Background: Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well.Recombinant human Fibroblast Growth Factor-6 (rhFGF-6) produced in E. coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-6 has a molecular mass of 18.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: Fibroblast Growth Factor-6, HBGF-6, HST-2

Molecular Weight: 18.8 kDa, observed by reducing SDS-PAGE.

UniProt: P10767

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway