FLT3LG Protein (AA 27-185)

Catalog No. ABIN2017866

Product Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-185

Origin: Human

Source: CHO Cells

Characteristics: ED50< 1 ng/mL, measured in a cell proliferation assay of human AML5 cells, corresponding to a specific activity of > 1 x 10^6 units/mg

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fms-related tyrosine kinase 3 ligand (Flt-3 Ligand) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFlt-3L should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt-3 Ligand (Flt-3L) (FLT3LG Products)

Background: Fms-related tyrosine kinase 3 ligand (Flt3L) is growth fator stimulates the proliferation and differentiation of hematopoietic multipotent progenitors and promotes proliferation of NK cells and dendritic cell subgroups by combination with other growth factors. Flt3L is produced by T cells and stromal fibroblasts, and targeted various cells including hematopoietic stem cells, B cells, T cells, dendritic cells, and NK cells [1][2]. Flt3L binds to it cognate tyrosine kinase receptor Flt3 and activates JAK/STAT signaling pathway[3].Flt3L is a hematopoietic four helical bundle cytokine with structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1) demonstrated four conserved cysteines and two glycosylation sites. Flt3L naturally as a non-disulfide-linked homodimer with multiple isoforms. The extracellular portion is approximately 160 amino acid residues in length and the cytoplasmic segment is approximately 20-30 amino acid residues in length[4].
Synonyms: Flt3 ligand, FL, Flt3LG, SL cytokine, Fms-related tyrosine kinase 3 ligand

Molecular Weight: Multiple bands between 18-22 kDa, observed by reducing SDS-PAGE.

Pathways: RTK Signaling