GM-CSF Protein

Catalog No. ABIN2017902

This Recombinant GM-CSF protein is expressed in Escherichia coli (E. coli) and has been mentioned in 7+ publications.

Quick Overview for GM-CSF Protein (ABIN2017902)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: Greater than 98 % as determined by(a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel

Product Details

Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN

Specificity: UV Scan: The maximal absorption wave is 279+/-3 nm.

Characteristics: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/mL, corresponding to a Specific Activity of 1.0x10^7 IU/mg.
LKDFLLVIPF DCWEPVQETh e sequence of the first fifteen N-terminal amino acids has been found to be (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.

Endotoxin Level: < 0.03 ng/μg (0.3 IEU/μg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that the lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Buffer: The protein was lyophilized after extensive dialysis against 20 mM Phosphate buffer, pH 7.0, 150 mM NaCl, 5 % Mannitol buffer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), although stable at room temperature for three weeks, should be stored desiccated at -18 °C or below. Upon reconstitution, Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) may be stored at 4 °C for short periods (two to seven days). For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added.

References

Mathew, Zaineb, Verma: "GM-CSF-DFF40: a novel humanized immunotoxin induces apoptosis in acute myeloid leukemia cells." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 7, pp. 882-95, (2013) (PubMed).

Wang, Thomson, Allan, Jackson, Olson, Hercus, Nero, Turner, Parker, Lopez, Waddell, Anderson, Hamilton, Schrader: "Characterization of pathogenic human monoclonal autoantibodies against GM-CSF." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7832-7, (2013) (PubMed).

Côté, Plante, Tardif, Tremblay: "Dectin-1/TLR2 and NOD2 agonists render dendritic cells susceptible to infection by X4-using HIV-1 and promote cis-infection of CD4(+) T cells." in: PLoS ONE, Vol. 8, Issue 7, pp. e67735, (2013) (PubMed).

Bouchlaka, Sckisel, Chen, Mirsoian, Zamora, Maverakis, Wilkins, Alderson, Hsiao, Weiss, Monjazeb, Hesdorffer, Ferrucci, Longo, Blazar, Wiltrout, Redelman, Taub, Murphy: "Aging predisposes to acute inflammatory induced pathology after tumor immunotherapy." in: The Journal of experimental medicine, Vol. 210, Issue 11, pp. 2223-37, (2013) (PubMed).

Allan, Stax, Zheng, Chung, Kozak, Tan, van den Elzen: "CD1d and CD1c expression in human B cells is regulated by activation and retinoic acid receptor signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 9, pp. 5261-72, (2011) (PubMed).

Lambert, Barabé, Gilbert, Tremblay: "DCIR-mediated enhancement of HIV-1 infection requires the ITIM-associated signal transduction pathway." in: Blood, Vol. 117, Issue 24, pp. 6589-99, (2011) (PubMed).

Ciesielski, Ahluwalia, Munich, Orton, Barone, Chanan-Khan, Fenstermaker: "Antitumor cytotoxic T-cell response induced by a survivin peptide mimic." in: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 8, pp. 1211-21, (2010) (PubMed).

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

Background: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils.Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.
Synonyms: rHuGM-CSF, GM-CSF

Molecular Weight: 14,000 Da

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin