GM-CSF Protein (AA 18-144)

Catalog No. ABIN2017905

Recombinant GM-CSF protein expressed in CHO Cells.

Quick Overview for GM-CSF Protein (AA 18-144) (ABIN2017905)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 18-144

Characteristics: ED50 < 0.2 ng/mL, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5x10^6 units/mg.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF)

Background: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effectors functions of granulocytes,monocytes/macrophages and eosinophils. Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.
Synonyms: Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-alpha

Molecular Weight: 22-28 kDa, observed by non-reducing SDS-PAGE.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin