Interferon gamma Protein (IFNG) (AA 23-156)

Catalog No. ABIN2018002

This Recombinant Interferon gamma protein is expressed in CHO Cells.

Quick Overview for Interferon gamma Protein (IFNG) (AA 23-156) (ABIN2018002)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: CHO Cells

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 23-156

Characteristics: ED50 < 2.5 ng/mL, measured by cytotoxicity assay using WEHI-279 cells, corresponding to a specific activity of > 4x10^5 units/mg.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant Rat Interferon gamma (IFN-gamma) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrIFN-gamma should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: Interferon-gamma (IFN-Gamma)

Background: Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The active form of IFN-gamma is an antiparallel dimer that interacts with the receptor IFN-gamma R1 and sets off IFN-gamma/JAK/STAT pathway. IFN-gamma signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-gamma induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-gamma is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.
Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF

Molecular Weight: 15-25 kDa, observed by non-reducing SDS-PAGE.

UniProt: P01581

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy