IL-21 Protein (AA 25-155)

Catalog No. ABIN2018094

Product Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 25-155

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 0.5 ng/mL, measured by a cell growth inhibition assay using Mino cells, corresponding to a specific activity of > 2.0x 10^6 units/mg.
AA 25-155, expressed with an N-terminal Met.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Interleukin-21 (rhIL-21) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-21 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: Interleukin-21(IL-21) (IL21 Products)

Background: Interleukin-21 (IL-21) belongs to the Type I four helix bundle cytokines, and shares the common cytokine receptor gamma chain with IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is expressed by CD4+ T cells, natural killer (NK) T cells, and Th17 cells, and the IL-21 receptor is highly expressed on CD4+ and CD8+ B cells, indeed, IL-21 plays a pivotal role in the survival and proliferation of B cells, and their differentiation to immunoglobulin (Ig) producing cells. IL-21 up-regulates and down-regulates the production of IgG1 and IgE by B cells, respectively, and diminishes the severity of allergy and asthma. In some case, IL-21 also induces the apoptosis of B cells. The other roles of IL-21 include regulation of innate immune systems, implication on autoimmunity, and antitumor actions.Recombinant human Interleukin-21 (rhIL-21) produced in E. coli is a single non-glycosylated polypeptide chain containing 132 amino acids. A fully biologically active molecule, rhIL-21 has a molecular mass of 15.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Za11

Molecular Weight: 15.4 kDa, observed by reducing SDS-PAGE.

UniProt: Q9HBE4

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response