FGF7 Protein (AA 32-194)

Catalog No. ABIN2018175

This Recombinant FGF7 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF7 Protein (AA 32-194) (ABIN2018175)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Product Details

Protein Characteristics: AA 32-194

Characteristics: ED50 < 2 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0x 10^5 units/mg.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse Keratinocyte Growth Factor (rmKGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmKGF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: Keratinocyte Growth Factor (KGF)

Background: Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF's roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney. Recombinant mouse Keratinocyte Growth Factor (rmKGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 163 amino acids. A fully biologically active molecule, rmKGF has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: Fibroblast Growth Factor-7, HBGF-7

Molecular Weight: 18.7 kDa, observed by reducing SDS-PAGE.

UniProt: P36363

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway