M-CSF/CSF1 Protein (AA 33-190, Isoform 3)

Catalog No. ABIN2018211

Recombinant M-CSF/CSF1 protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for M-CSF/CSF1 Protein (AA 33-190, Isoform 3) (ABIN2018211)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by non-reducing SDS-PAGE.

Product Details

Protein Characteristics: Isoform 3, AA 33-190

Characteristics: ED50 of 1 - 3 ng/mL, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 10^6 units/mg.
AA 33-190 (Isoform 3), expressed with an N-terminal Met.

Endotoxin Level: < 1 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Expiry Date: 6 months

References

Bertin, Barat, Bélanger, Tremblay: "Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells." in: Journal of neuroinflammation, Vol. 9, pp. 55, (2012) (PubMed).

Target Details

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Macrophage Colony Stimulating Factor (M-CSF)

Background: Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63. Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Molecular Weight: 28 kDa, observed by non-reducing SDS-PAGE.

UniProt: P09603

Pathways: RTK Signaling