Neurotrophin 4 Protein (NTF4) (AA 80-209)

Catalog No. ABIN2018283

Recombinant Neurotrophin 4 protein expressed in Escherichia coli (E. coli).

Quick Overview for Neurotrophin 4 Protein (NTF4) (AA 80-209) (ABIN2018283)

Target: Neurotrophin 4 (NTF4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGEanalysis.

Product Details

Protein Characteristics: AA 80-209

Characteristics: ED50 <1 μg/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of>1× 10^3 units/mg.
AA 80-209, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in 50 mM acetic acid or ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM acetic acid.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: Neurotrophin 4 (NTF4)

Alternative Name: Neurotrophin-4 (NT-4)

Background: Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NF-kappaB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer's.Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E. coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0 kDaanalyzed by reducing SDS-PAGE.
Synonyms: Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5)

Molecular Weight: 14.0 kDa, observed by reducing SDS-PAGE.

UniProt: Q80VU4

Pathways: RTK Signaling