Oncostatin M Protein (OSM) (AA 26-252)

Catalog No. ABIN2018292

This Recombinant Oncostatin M protein is expressed in Escherichia coli (E. coli).

Quick Overview for Oncostatin M Protein (OSM) (AA 26-252) (ABIN2018292)

Target: Oncostatin M (OSM)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE analysis.

Product Details

Protein Characteristics: AA 26-252

Characteristics: ED50 < 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1x 10^5 units/mg.
AA 26-252, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Oncostatin M (rhOSM) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: Oncostatin M (OSM)

Background: Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and share a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor beta or LIF Receptor beta to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. On the other hand, OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.Recombinant human Oncostatin M (rhOSM) produced in E. coli is a single non-glycosylated polypeptide chain containing 228 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 25.9 kDa analyzed by reducing SDS-PAGE.
Synonyms: OSM

Molecular Weight: 25.9 kDa, observed by reducing SDS-PAGE.

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion