Oncostatin M Protein (OSM) (AA 26-221) (His-Avi Tag)
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- Target See all Oncostatin M (OSM) Proteins
- Oncostatin M (OSM)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-221
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Oncostatin M protein is labelled with His-Avi Tag.
- Purpose
- Human Oncostatin M/OSM Protein
- Sequence
- Ala26-Arg221
- Characteristics
- Recombinant Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Arg221.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Oncostatin M (OSM) (Active) protein
OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Oncostatin M (OSM) (AA 25-206) protein (His tag)OSM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Oncostatin M (OSM) protein (His-Avi Tag,Biotin)OSM Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Oncostatin M (OSM) (AA 26-239) protein (His tag)OSM Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Oncostatin M (OSM) (AA 26-221) protein (His tag)OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Oncostatin M (OSM) (AA 26-234) protein (His tag)OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Oncostatin M (OSM) (AA 1-252) protein (GST tag)OSM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Oncostatin M (OSM) (AA 25-206) protein (His tag)Crystallography grade OSM Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Oncostatin M (OSM) (AA 26-221) protein (His tag)Crystallography grade OSM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Oncostatin M (OSM)
- Alternative Name
- OSM (OSM Products)
- Synonyms
- OSM Protein, OncoM Protein, oncostatin M Protein, OSM Protein, Osm Protein
- Background
- OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
- Molecular Weight
- 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
- UniProt
- P13725
- Pathways
- JAK-STAT Signaling, Negative Regulation of Hormone Secretion
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