PDGF-BB Homodimer (AA 82-190) (Active) Protein
Origin: Mouse
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS-PAGE analysis.
Active
-
- Target
- PDGF-BB Homodimer
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 82-190
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Characteristics
-
ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4 x 10^5 units/mg.
AA 82-190, expressed with an N-terminal Met. - Purity
- > 95 % by SDS-PAGE analysis.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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PDGF-BB Homodimer (Active) protein (His tag)
Origin: Human Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer (AA 82-190) (Active) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
-
- Target
- PDGF-BB Homodimer
- Alternative Name
- Platelet-Derived Growth Factor-BB (PDGF-BB)
- Synonyms
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
- Background
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Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: PDGF-2, GDGF, ODGF, SIS, SSV - Molecular Weight
- 24.7 kDa, observed by non-reducing SDS-PAGE.
- UniProt
- P31240
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