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KIT Ligand Protein (KITLG) (AA 26-189)

This Recombinant KIT Ligand protein is produced in Yeast (Pichia pastoris).
Catalog No. ABIN2018383

Quick Overview for KIT Ligand Protein (KITLG) (AA 26-189) (ABIN2018383)

Target

See all KIT Ligand (KITLG) Proteins
KIT Ligand (KITLG)

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 28
  • 17
  • 11
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Rat

Source

  • 37
  • 9
  • 8
  • 8
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Yeast (Pichia pastoris)

Purity

> 95 % by SDS-PAGE analysis.
  • Protein Characteristics

    AA 26-189

    Characteristics

    ED50 < 25 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 4x 10^4 units/mg.

    Endotoxin Level

    < 0.2 EU/μg, determined by LAL method.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstituted in ddH2O at 100 μg/mL.

    Buffer

    Lyophilized after extensive dialysis against PBS.

    Storage

    -80 °C

    Storage Comment

    Lyophilized recombinant rat Stem Cell Factor (rrSCF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrSCF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

    Expiry Date

    6 months
  • Target

    KIT Ligand (KITLG)

    Alternative Name

    Stem Cell Factor (SCF)

    Background

    Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant rat Stem Cell Factor (rrSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 164 amino acids. A fully biologically active molecule, rrSCF has a molecular mass of 18.3 kDa analyzed by reducing SDS-PAGE.
    Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

    Molecular Weight

    18.3 kDa, observed by reducing SDS-PAGE.

    UniProt

    P21581

    Pathways

    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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